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KMID : 0350519940470020959
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Journal of Catholic Medical College
1994 Volume.47 No. 2 p.959 ~ p.969
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The Effect and the Synergistic Effect of Photodynamic Therapy in Combination with Thiotepa or Adriamycin in a Murine Bladder Tumor Model
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Abstract
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Photodynamic therapy(PDT) has been used for the treatment of a variety of tumors and currently is under investigation as form of therapy for the superficial bladder cancer.
The precise mechanism of PDT is not known, however, two actions by which PDT causes tumor destruction have been documented. The first is a direct cytotoxic effect by production of singlet oxygen and free redicals. The second is through vascular
endothelial damage and subsequent hypoxia and tissue death.
Recently, immunologic involvement in cytotoxicity by PDT has been implicated.
In addition, there are few reports of the interaction between PDT and anticancer chemotherapeutic agents in animal tumor models, but the efficacy of PDT combined with the agents that are currently used for the treatment of bladder cancer has not
been
rigorously evaluated in a vivo system.
This study was designed to evaluate 1) tumoricidal effect and immunologic response following PDT, and 2) the interaction of PDT and Thiotepa or Adriamycin, and immunologic response following PDT in combination with the drugs in an animal bladder
tumor
model.
C3H/HeN mine with implanted NBT-2 tumors were treated with either Thiotepa, Adriamycin, PDT or PDT in combination with the drugs.
@ES The results were as follows:
@EN 1. Tumor growth was significantly inhibited by either PDT, PDT combined with Thiotepa or Adriamycin compared to other groups from one to four weeks following treatment(p<0.05). PDT combined with Adriamycin was significantly effective in
inhibiting
tumor growth compared to either PDT or PDT combined with Thiotepa from three to four weeks following treatment(P<0.05).
2. Ten percent of the tumors treated by either PDT, PDT combined with Thiotepa or Adriamycin were tumor free(oure) at four weeks following treatment but, no significant differences in cure rate were seen between these cured group and other
groups.
3. Natural killer(NK) activity against YAC-1 target cells was significnatly inhibited by PDT combined with Thiotepa compared to other groups on third day following treatment(P<0.05).
4. Phagocytic activity of mononuclear cells was significantly inhibited by PDT compared to other groups on third day following treatment(P<0.05).
5. No significnat differences in CD4/CD8 ratio of peripheral blood lymphocyte were seen between all experimental groups.
This study demonstrates that PDT is significantly effective in inhibiting tumor growth compared to either Thiotepa or Adriamycin alone without significant immunologic impairment and certaincombination of PDT and anticancer drug such as Adriamycin
may
results in enhanced tumoricidal effect witout immunologic impairment compared to PDT alone.
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KEYWORD
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